Asian Research Journal of Current Science

Bulbophyllum auricomum is a royal orchid of Myanmar people due to its remarkable value and sweet fragrance. Because of notably limited in the growth and propagation, and also due to over exploitation and habitat destruction, it becomes now an endangered species. The aim of this study was to study the effect of plant growth regulators and organic additives on multiplication of B. auricomum. The research was conducted at tissue culture laboratory of Htone Bo Farm, DAR, Taunggyi, Shan State, Myanmar. In vitro germinated seedlings were used as the plant materials for shoot regeneration. The experimental design was two factors factorial arrangement in RCB design with factor A of different levels of 6-benzyl amino purine (BAP) and Kinetin (Kin) (1,3 mg L^-1) in combination with 0.50 mg L^-1NAA, and organic additives of potato, rice, banana each 100 g L^-1 and factor B of Kayin, Dawei and Rakhine seedlings. Statistix version 8.0 and mean comparisons were performed using LSD at the 5% level. From this study, the organic additives fortified treatments shown the superior effects on shoot regeneration of selected B. auricomum cultivars. The medium each fortified with 100g L-¹ banana gave maximum number of shoots per explant and followed by 100 g L^-1 rice 15.33 shoots per explant and 13.00 on 100 g L^-1 potato. Twenty weeks after inoculation roots and bulbs were well developed.  100g L^-1 potato supplement medium produced the maximum number of roots (8.33) and (7.58) on 100 g L^-1 rice fortified medium, followed by that of banana accomplished medium (5.66). The incorporation of organic additives into culture media significantly enhanced in vitro shoot growth of B. auricomum, demonstrating its potential for commercial production. Extensive field trials are still required to assess the long-term survival and flowering consistency of the acclimatized plants.